Milev N
Vet Med Nauki. 1975;12(4):95-9.
In the treatment of spleens and lumph nodes of rabbits infected with strain K, with the use of freon 113 the virus was freed from proteins up to 92-96 per cent. The infective power of the purified virus proved equal to that of the initial non-purified virus proved equal to that of the initial non-purified virus, and this points to the full extraction of the virus itself. The antigenic properties of the purified virus were demonstrated through agar gel precipitation with normal and hyperimmune sera of pigs and rabbits and indirect immunofluorescence methods. This method of purification yields a virus that can be used in more precise immunologic studies as well as in the study of the morphology of the classic swine fever virus.
在用氟利昂113处理感染K株的兔脾脏和淋巴结时,病毒中的蛋白质被去除达92%至96%。纯化病毒的感染力经证实与初始未纯化病毒的相等,这表明病毒本身已被完全提取。通过用猪和兔的正常血清及超免疫血清进行琼脂凝胶沉淀试验以及间接免疫荧光法,证明了纯化病毒的抗原特性。这种纯化方法得到的病毒可用于更精确的免疫学研究以及经典猪瘟病毒形态学的研究。