Hotter G, Ramis I, Closa D, Roselló-Catafau J, Gelpí E
Molecular Pathology Unit, CID-CSIC, Barcelona, Spain.
Agents Actions. 1993 Sep;40(1-2):72-7. doi: 10.1007/BF01976754.
The differentiation of HL-60 human promyelocytic leukaemia cells into specific monocytic or granulocytic lineage cells depending of the inductor agent is accompanied by selective regulation of several key enzymes involved in the synthesis of eicosanoids. In this communication we have investigated the changes in arachidonic acid metabolic profiles during phorbol 12-myristate 13-acetate (PMA)-induced monocytic differentiation of HL-60 cells. Our results show that HL-60 cells have spontaneous capacity to synthesize large amounts of LTB4, but PMA-differentiated cells lose the ability to release LTB4. Significant differences are found between HL-60 cells and PMA-treated cells in basal conditions and under ionophore stimulation. The addition of LTB4 at the time of PMA differentiation did not have effects on cell proliferation, but nordihydroguaiaretic acid (NDGA), a potent 5-lipoxygenase inhibitor, also inhibited HL-60 cell proliferation and did not have any effect on PMA-differentiated cell proliferation.
HL-60人早幼粒细胞白血病细胞根据诱导剂的不同分化为特定的单核细胞或粒细胞系细胞，这一过程伴随着参与类花生酸合成的几种关键酶的选择性调节。在本通讯中，我们研究了佛波酯12-肉豆蔻酸酯13-乙酸酯（PMA）诱导HL-60细胞单核细胞分化过程中花生四烯酸代谢谱的变化。我们的结果表明，HL-60细胞具有自发合成大量白三烯B4（LTB4）的能力，但经PMA分化的细胞失去了释放LTB4的能力。在基础条件下和离子载体刺激下，HL-60细胞与经PMA处理的细胞之间存在显著差异。在PMA分化时添加LTB4对细胞增殖没有影响，但去甲二氢愈创木酸（NDGA），一种有效的5-脂氧合酶抑制剂，也抑制HL-60细胞增殖，而对经PMA分化的细胞增殖没有任何影响。
