Bowles J, Hope M, Tiu W U, Liu X, McManus D P
Molecular Parasitology Unit, Queensland Institute of Medical Research, Brisbane, Australia.
Acta Trop. 1993 Dec;55(4):217-29. doi: 10.1016/0001-706x(93)90079-q.
Geographical isolates of S. japonicum, and particularly isolates from China and the Philippines, were examined at the molecular level for genetic divergence. Sequences from both nuclear and mitochondrial genomes were selected as markers of evolutionary divergence and S. mekongi and S. mansoni were included in the study for comparison purposes. Restriction fragment length polymorphism (RFLP) and PCR-RFLP analysis of the rDNA repeat unit and sequence analysis of the second internal transcribed spacer region (ITS2) within the rDNA repeat and the cytochrome c oxidase I (COI) gene of the mitochondrial genome were performed. No intra-specific variation in S. japonicum was found in the rDNA repeat and only very slight variation was detected within the COI sequence. A survey of the entire genome, using random amplified polymorphic DNA (RAPD) analysis, again showed that Chinese and Philippine S. japonicum are remarkably similar at the DNA sequence level. We were thus unable to obtain direct molecular evidence in support of previous findings, particularly those based on isoenzyme analysis, that a very high level of intra-specific variation exists in S. japonicum.
对日本血吸虫的地理隔离株，特别是来自中国和菲律宾的隔离株，进行了分子水平的遗传分化研究。选择核基因组和线粒体基因组的序列作为进化分化的标记，并将湄公血吸虫和曼氏血吸虫纳入研究以作比较。对核糖体DNA（rDNA）重复单元进行了限制性片段长度多态性（RFLP）和PCR-RFLP分析，并对rDNA重复序列中的第二内部转录间隔区（ITS2）以及线粒体基因组的细胞色素c氧化酶I（COI）基因进行了序列分析。在rDNA重复序列中未发现日本血吸虫种内变异，在COI序列中仅检测到非常轻微的变异。使用随机扩增多态性DNA（RAPD）分析对整个基因组进行的一项调查再次表明，中国和菲律宾的日本血吸虫在DNA序列水平上非常相似。因此，我们无法获得直接的分子证据来支持先前的研究结果，特别是那些基于同工酶分析的结果，即日本血吸虫存在非常高水平的种内变异。
