Carughi A, Hooper F G
Neo-Life Company of America, Fremont, CA 94537-5012.
Am J Clin Nutr. 1994 Apr;59(4):896-9. doi: 10.1093/ajcn/59.4.896.
Plasma carotenoid concentrations were determined by HPLC in 11 individuals consuming low-carotenoid diets and after taking a carotenoid supplement. Subjects first consumed low-carotenoid diets for 2 wk, then supplemented these diets daily with 8.5 mg beta-carotene, 3.5 mg alpha-carotene, and 0.5 mg lycopene, from natural sources for 4 wk. Serum cholesterol, triglycerides, and lipoproteins were determined before and after supplementation. After 2 wk on the low-carotenoid diet, plasma concentrations of the three carotenoids fell to approximately 60% of baseline values. One week after supplementation, alpha- and beta-carotene concentrations returned to baseline and by the end of the supplementation period they were significantly higher than baseline values (P < 0.05). Lycopene concentrations increased only slightly. Serum lipids did not change significantly. Overall, plasma concentrations of these carotenoids reflect the amount provided by the supplement. This is the first study reporting increments of serum carotenoids, other than beta-carotene, after supplementation.
通过高效液相色谱法（HPLC）测定了11名食用低类胡萝卜素饮食的个体以及服用类胡萝卜素补充剂后的血浆类胡萝卜素浓度。受试者首先食用低类胡萝卜素饮食2周，然后在接下来的4周内每天从天然来源补充8.5毫克β-胡萝卜素、3.5毫克α-胡萝卜素和0.5毫克番茄红素。在补充前后测定血清胆固醇、甘油三酯和脂蛋白。在低类胡萝卜素饮食2周后，三种类胡萝卜素的血浆浓度降至基线值的约60%。补充1周后，α-和β-胡萝卜素浓度恢复到基线水平，到补充期结束时，它们显著高于基线值（P<0.05）。番茄红素浓度仅略有增加。血清脂质没有显著变化。总体而言，这些类胡萝卜素的血浆浓度反映了补充剂提供的量。这是第一项报告补充后除β-胡萝卜素外血清类胡萝卜素增加的研究。
