Pflüger R, Scharmann W, Blobel H
Zentralbl Bakteriol Orig A. 1975 Oct;233(2):236-44.
In an effort to learn more about the pathogenicity of Pseudomonas aeruginosa we studied the culture supernatants of 20 strains of this bacterial species. Of these 5 proved to be highly toxic, 9 moderately toxic and 6 nontoxic for mice. The toxic component could not be separated from protease (elastase), neither by gel-filtration on Sephadex G-100 superfine, nor by isoelectric focusing. From this we assumed that the toxicity of the culture supernatants was associated with elastase. Purified elastase from P. aeruginosa, strain 75, elicited cytotoxic effects in cultures of L-, HeLa- and testicular-cells from calves. It apparently did not damage cultures of kidney-cells from rabbits and pigs.
为了更多地了解铜绿假单胞菌的致病性，我们研究了该细菌物种20个菌株的培养上清液。其中5个菌株对小鼠具有高毒性，9个菌株具有中等毒性，6个菌株无毒。毒性成分无法通过在Sephadex G-100超细微粒上进行凝胶过滤或等电聚焦从蛋白酶（弹性蛋白酶）中分离出来。由此我们推测培养上清液的毒性与弹性蛋白酶有关。从铜绿假单胞菌75菌株中纯化得到的弹性蛋白酶对小牛的L细胞、HeLa细胞和睾丸细胞培养物产生细胞毒性作用。它显然不会损害兔和猪的肾细胞培养物。
