Gestrich R, Mehlhorn H, Heydorn A O
Zentralbl Bakteriol Orig A. 1975 Oct;233(2):261-76.
In several experiments young calves were infected with oocyss and sporocysts of the large form of isospora bigemina from cats, which has been fed by raw beef containing Sarcocystis fusiformis cysts. On he 98th and 160th day p.i. the calves were killed and the development of S. fusiformis cysts in muscle cells was sutided by lign and electron microscopy. The cyst was always situated within a muscle fiber which was never surrounded by fibrillar layers of host origin (= no secondary cyst wall). The cyst was limited by a singel unit membrane, which was thickened at numerous places of the interior by osmiophilic material. This complex is called primary wall (Primärhülle), reaching a thickness of up to 250 A. This primary wall was regulary folded, forming palisade-like protrusions...
在几个实验中,用来自猫的大型双芽伊氏孢子虫的卵囊和子孢子感染幼小牛,这些猫被喂食了含有梭形肉孢子虫囊肿的生牛肉。在感染后第98天和160天,宰杀小牛,通过光学显微镜和电子显微镜研究肌肉细胞中梭形肉孢子虫囊肿的发育情况。囊肿总是位于肌纤维内,肌纤维从未被宿主来源的纤维层包围(=无次生囊壁)。囊肿由单层单位膜限制,该膜在内部许多地方被嗜锇物质增厚。这种复合体称为初级壁(Primärhülle),厚度可达250埃。初级壁规则折叠,形成栅栏状突起……