Stroetmann I, Kämpfer P, Dott W
Fachgebiet Hygiene der Technischen Universität Berlin.
Zentralbl Hyg Umweltmed. 1994 Jan;195(2):111-20.
Three different soil types (two sandy materials and one till soil type) were sterilized with 14 different sterilization methods or variations. The cation-exchange capacity and specific surface of the soil samples were chosen as control parameters of physico-chemical modifications in the soils. Incubation of soil samples in the presence of Na-azide and HgCl2, in addition to tyndallization and fractionated heating of soil lead to a significant reduction of microorganisms. UV radiation and alternating freezing and thawing of soil had no influence on the number of microorganisms. The application of mercury-II-chloride did not lead to the expected sterilization, especially with till. A sterilization of soil is possible by using formaldehyde and ethylene oxide gassing, in addition to autoclaving and gamma radiation. No microorganisms could be detected in the soils after application of these sterilization methods. The cation exchange capacity of the soils was only slightly influenced with these methods. The use of gamma radiation seems to be the best method for sterilization of soils.
三种不同的土壤类型(两种砂质材料和一种冰碛土类型)采用14种不同的灭菌方法或变体进行了灭菌处理。选择土壤样品的阳离子交换容量和比表面积作为土壤物理化学改性的控制参数。除了间歇灭菌和土壤分级加热外,在叠氮化钠和氯化汞存在下对土壤样品进行培养会导致微生物数量显著减少。紫外线辐射以及土壤的交替冻融对微生物数量没有影响。二氯化汞的应用并未导致预期的灭菌效果,尤其是对于冰碛土。除了高压灭菌和伽马辐射外,使用甲醛和环氧乙烷熏蒸也可以对土壤进行灭菌。应用这些灭菌方法后,土壤中未检测到微生物。这些方法对土壤的阳离子交换容量影响很小。伽马辐射似乎是土壤灭菌的最佳方法。