Nishimura K, Matsumoto T, Naito M, Noguchi Y, Shigeyama Y, Kitamura H, Hayashi M, Amatsu S, Matsushita K, Yamaoka A
Department of Periodontics, Osaka Dental University.
Hum Cell. 1993 Dec;6(4):300-9.
The purpose of this study was two-fold; first to investigate the chemotactic response of periodontal cells to root surface components during wound healing following flap surgery; second, to investigate the chemoattractive effect of the root components on cell migration. Cells were obtained by culturing cells from monkey periodontal tissues and rat calvarial cells. Root surface components were obtained from teeth extracted at 4, 7, 10 and 14 days after flap surgery. The chemotactic response of the isolated cells to root surface components was quantitated by an in vitro assay using a 48 well microchemotaxis chamber. The effect of root components or other growth factors on the cell migration was evaluated using a three-dimensional gel system. Cemental components produced a strong chemotactic response for gingival fibroblasts, alveolar bone cells and rat calvarial cells. Kinetics of the chemotactic potential of cementum was characterized by an initial increase (up to 7 days after surgery), a transient decline (at 10 days after surgery) and a second increase (at 14 days after surgery). Cemental components obtained 7 days after surgery most profoundly facilitated invasion of gingival fibroblasts with comparable effects induced by PDGF and TGF-beta. These results suggest that cemental components influence the chemotactic migration of gingival fibroblasts at the initial stage of wound healing, and therefore, cementum plays an important role in periodontal regeneration.
本研究目的有两个；其一，研究翻瓣手术后伤口愈合过程中牙周细胞对根面成分的趋化反应；其二，研究根成分对细胞迁移的化学吸引作用。通过培养猴牙周组织细胞和大鼠颅骨细胞获取细胞。根面成分取自翻瓣手术后4天、7天、10天和14天拔除的牙齿。使用48孔微量趋化室通过体外试验对分离细胞对根面成分的趋化反应进行定量。使用三维凝胶系统评估根成分或其他生长因子对细胞迁移的影响。牙骨质成分对牙龈成纤维细胞、牙槽骨细胞和大鼠颅骨细胞产生强烈的趋化反应。牙骨质趋化潜能的动力学特征为初期升高（术后7天内）、短暂下降（术后10天）和再次升高（术后14天）。术后7天获取的牙骨质成分最显著地促进了牙龈成纤维细胞的侵入，其效果与血小板衍生生长因子（PDGF）和转化生长因子-β（TGF-β）诱导的效果相当。这些结果表明，牙骨质成分在伤口愈合初期影响牙龈成纤维细胞的趋化迁移，因此，牙骨质在牙周再生中起重要作用。
