Ogata Y, Niisato N, Moriwaki K, Yokota Y, Furuyama S, Sugiya H
Department of Periodontology, Nihon University School of Dentistry at Matsudo, Chiba, Japan.
Comp Biochem Physiol B Biochem Mol Biol. 1997 Mar;116(3):359-65. doi: 10.1016/s0305-0491(96)00255-6.
In this paper, we used the extracts from cementum, bone, dentin and enamel, and compared the chemotactic behavior of human periodontal ligament (HPDL) cells, human gingival fibroblasts (HGF) and human alveolar bone (HAB) cells using modified Boyden chambers. The extracts, obtained using 4 M guanidine HCl ("G" extract), from cementum, root dentin, and bone had greater chemotactic effects than all the 4 M guanidine HCl/0.5 M EDTA extracts ("E" extract). HPDL cells and HGF exhibited higher chemotactic behavior than osteoblast-like HAB cells in response to cementum and root dentin "G" extracts. On the other hand, HAB cells showed the highest migratory activity in response to the bone "G" extract. The chemotactic activities of these extracts were reduced by heat- and trypsin-treatment. These results suggest that proteinaceous chemotactic factors exist in the cementum, root dentin and bone, and they appear to regulate the migration and orientation of HPDL cells, HGF and HAB cells during periodontal wound healing.
在本文中，我们使用了来自牙骨质、骨、牙本质和釉质的提取物，并使用改良的博伊登小室比较了人牙周膜（HPDL）细胞、人牙龈成纤维细胞（HGF）和人牙槽骨（HAB）细胞的趋化行为。使用4M盐酸胍（“G”提取物）从牙骨质、牙根牙本质和骨中获得的提取物比所有4M盐酸胍/0.5M乙二胺四乙酸提取物（“E”提取物）具有更强的趋化作用。在对牙骨质和牙根牙本质“G”提取物的反应中，HPDL细胞和HGF表现出比成骨样HAB细胞更高的趋化行为。另一方面，HAB细胞对骨“G”提取物表现出最高的迁移活性。这些提取物的趋化活性通过加热和胰蛋白酶处理而降低。这些结果表明，牙骨质、牙根牙本质和骨中存在蛋白质趋化因子，它们似乎在牙周伤口愈合过程中调节HPDL细胞、HGF和HAB细胞的迁移和定向。
