Griffiths W C, Bogaars H A, Sheehan C F, Diamond B S
Ann Clin Lab Sci. 1976 Mar-Apr;6(2):177-83.
The technique of acrylamide gel electrophoresis of sodium dodecyl sulfate treated protein mixtures has been applied to the analysis of human serum proteins in the 70,000 to 250,000 molecular weight range. After staining, the bands are well defined, the molecular weight is defined and, hence, the identity of each can be estimated from the migration distance. In ambiguous cases, the identification of a band is confirmed by an independent method. The procedure is particularly valuable in the study of the gammopathies. The immunoglobulins migrate as expected, in contrast to the problems often encountered in the Ornstein-Davis gel method. Furthermore, light chains migrate more rapidly, are well separated from other serum proteins and, therefore, are readily detected. Paraproteins from three patients with documented gammopathies have been studied and characterized using this method.
十二烷基硫酸钠处理的蛋白质混合物的丙烯酰胺凝胶电泳技术已应用于分析分子量在70,000至250,000范围内的人血清蛋白。染色后，条带清晰，分子量明确，因此，每条带的身份可根据迁移距离估算。在不明确的情况下，通过独立方法确认条带的身份。该方法在丙种球蛋白病的研究中特别有价值。与奥恩斯坦 - 戴维斯凝胶法中经常遇到的问题相反，免疫球蛋白按预期迁移。此外，轻链迁移更快，与其他血清蛋白分离良好，因此易于检测。使用该方法对三名有记录的丙种球蛋白病患者的副蛋白进行了研究和表征。
