Wintersteiger R, Ofner B, Juan H, Windisch M
Institute of Pharmaceutical Chemistry, Karl-Franzens-University Graz, Austria.
J Chromatogr A. 1994 Feb 4;660(1-2):205-10. doi: 10.1016/0021-9673(94)85114-X.
A liquid chromatographic method for the determination of pyrethrins and of the synergist piperonyl butoxide in human plasma after C18 solid-phase extraction is described. UV detection was found to be sensitive enough to determine concentrations far below the limit of toxicity. With respect to future investigations concerning studies in biological materials, a column-switching system for sample preparation was developed and compared with solid-phase extraction. Both methods show comparable limits of detection, but the column-switching technique has the advantage of fully automating the system.
描述了一种在进行C18固相萃取后测定人血浆中除虫菊酯和增效剂胡椒基丁醚的液相色谱方法。发现紫外检测灵敏度足以测定远低于毒性限度的浓度。针对未来有关生物材料研究的调查，开发了一种用于样品制备的柱切换系统，并与固相萃取进行了比较。两种方法的检测限相当，但柱切换技术具有使系统完全自动化的优势。
