Pennsylvania Animal Diagnostic Laboratory System Toxicology Laboratory, New Bolton Center, University of Pennsylvania School of Veterinary Medicine, 382 W Street Rd., Kennett Square, PA 19348, USA.
Toxins (Basel). 2023 Jun 17;15(6):401. doi: 10.3390/toxins15060401.
The presence of insecticides like pyrethrins and synthetic pyrethroids, combined with the synergist piperonyl butoxide, in animal feeds can pose a risk to both animal and human health by contaminating the food chain. In this study, a simple and fast method was developed for the simultaneous determination of these compounds in contaminated animal feeds using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Sample preparation was carried out using a QuEChERS-based approach, and the method was validated with acceptable accuracy ranging from 84 to 115% and precision below 10%. The limit of detection (LOD) and limit of quantification (LOQ) were between 0.15 and 3 and 1 and 10 µg/kg, respectively. The method detected insecticide contaminations in various livestock and poultry feeds. Furthermore, the method was applied to a toxicology case, where it identified and quantified piperonyl butoxide and deltamethrin in the submitted horse feed sample. These results demonstrate that the method can be a valuable tool in animal health and food safety diagnostic applications, as well as veterinary toxicology investigations concerning pyrethrin-related feed contamination.
在动物饲料中存在杀虫剂如除虫菊酯和合成拟除虫菊酯，以及增效剂胡椒基丁醚，这可能会通过污染食物链对动物和人类健康造成风险。在这项研究中，开发了一种使用液相色谱-串联质谱法（LC-MS/MS）同时测定这些化合物在污染动物饲料中的简单快速方法。使用基于 QuEChERS 的方法进行样品制备，该方法的验证具有可接受的准确度范围为 84%至 115%和低于 10%的精密度。检测限（LOD）和定量限（LOQ）分别在 0.15 至 3 和 1 至 10 µg/kg 之间。该方法检测到各种牲畜和家禽饲料中的杀虫剂污染。此外，该方法还应用于毒理学案例，在提交的马饲料样品中鉴定和定量了胡椒基丁醚和溴氰菊酯。这些结果表明，该方法可以成为动物健康和食品安全诊断应用以及兽医毒理学中与拟除虫菊酯相关的饲料污染调查的有用工具。
