Durak I, Ormeci N, Akyol O, Canbolat O, Kavutçu M, Bülbül M
Ankara University, Faculty of Medicine, Department of Biochemistry, Turkey.
Dig Dis Sci. 1994 Apr;39(4):721-8. doi: 10.1007/BF02087413.
Adenosine deaminase (ADA), 5'-Nucleotidase (5NT), Xanthine oxidase (XO), Cu-Zn Superoxide dismutase (SOD) and Catalase (CAT) activities were determined in gastric juices from patients with gastric cancer, ulcer, gastritis and from healthy subjects. Enzyme activities were given as units per ml gastric juice and units per mg protein in gastric juice. ADA, 5NT and XO activities were found lower and protein concentrations were found higher in the cancer group than controls. There was however no significant difference between Cu-Zn SOD activities of the cancer and control groups. In all groups including control one, we could not find catalase activities in most of the samples. On the other hand, ADA, 5NT activities and protein concentrations in the gastric juice were lower in the gastritis group than control group. In the ulcer group, we found higher Cu-Zn SOD and XO activities and lower 5NT activity and protein concentrations compared with control values. In an attempt to establish statistical correlations between mean enzyme activities, pH and protein concentrations in the gastric juices of the groups, we found noticeable intra and inter-correlations, which indicated possible relations between DNA and free radical metabolizing enzymes.
测定了胃癌、溃疡、胃炎患者以及健康受试者胃液中的腺苷脱氨酶（ADA）、5'-核苷酸酶（5NT）、黄嘌呤氧化酶（XO）、铜锌超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性。酶活性以每毫升胃液的单位数和每毫克胃液蛋白质的单位数表示。发现癌症组的ADA、5NT和XO活性低于对照组，而蛋白质浓度高于对照组。然而，癌症组和对照组的铜锌SOD活性之间没有显著差异。在包括对照组在内的所有组中，我们在大多数样本中未发现过氧化氢酶活性。另一方面，胃炎组胃液中的ADA、5NT活性和蛋白质浓度低于对照组。与对照值相比，溃疡组中我们发现铜锌SOD和XO活性较高，而5NT活性和蛋白质浓度较低。为了在各组胃液中的平均酶活性、pH值和蛋白质浓度之间建立统计相关性，我们发现了显著的组内和组间相关性，这表明DNA与自由基代谢酶之间可能存在关联。
