Walker J D, Colwell R R, Petrakis L
Can J Microbiol. 1976 Mar;22(3):423-8. doi: 10.1139/m76-063.
Chesapeake Bay sediment bacteria from oil-contaminated and oil-free environments were compared for their ability to utilize a South Louisiana crude oil. Preferential solubility, column chromatography, gas-liquid chromatography, and computerized mass spectrometry were used to provide new and useful information regarding biodegradation of fractions and components of the crude oil. Vibrio, Pseudomonas, and Acinetobacter spp. were isolated from the culture inoculated with oil-contaminated sediment, whereas coryneforms and Pseudomonas spp. were isolated from the culture inoculated with oil-free sediment. Microorganisms from the oil-free sediment produced greater quantities of polar n-pentane-insoluble components (asphaltenes) after degradation, whereas microorganisms from the oil-contaminated sediments provided greater degradation of saturated and aromatic hydrocarbons.
对切萨皮克湾受石油污染和未受石油污染环境中的沉积物细菌利用路易斯安那州南部原油的能力进行了比较。采用优先溶解度、柱色谱、气液色谱和计算机质谱法，以提供有关原油馏分和成分生物降解的新的有用信息。从接种了受石油污染沉积物的培养物中分离出弧菌属、假单胞菌属和不动杆菌属，而从接种了未受石油污染沉积物的培养物中分离出棒状杆菌属和假单胞菌属。未受石油污染沉积物中的微生物在降解后产生了更多的极性正戊烷不溶成分（沥青质），而受石油污染沉积物中的微生物对饱和烃和芳烃的降解作用更强。
