Vials A J, Burnstock G
Department of Anatomy and Developmental Biology, University College London, UK.
Eur J Pharmacol. 1994 Jan 14;251(2-3):299-302. doi: 10.1016/0014-2999(94)90413-8.
The effect of suramin, a P2 purinoceptor antagonist, on the vasodilator response to adenosine 5'-triphosphate (ATP), 2-methylthio-ATP (2-meSATP) and adenosine were examined in the Sprague-Dawley rat coronary vasculature using the Langendorff heart preparation. Relaxation induced by 2-meSATP was significantly inhibited by suramin. Only responses to low doses of adenosine and ATP were inhibited by suramin. 8-(p-Sulphophenyl)theophylline (8-PSPT) did not affect the relaxant response to ATP and 2-meSATP at a concentration that significantly inhibited the response to adenosine. It is concluded that 2-meSATP acts via P2Y purinoceptors while ATP appears to be acting largely through a different mechanism. It is not acting via a P1 purinoceptor because ATP was not inhibited by the P1 purinoceptor antagonist 8-PSPT.
使用Langendorff心脏制备法，在斯普拉格-道利大鼠冠状动脉血管系统中研究了P2嘌呤受体拮抗剂苏拉明对血管舒张剂对5'-三磷酸腺苷（ATP）、2-甲硫基-ATP（2-meSATP）和腺苷反应的影响。苏拉明显著抑制了2-meSATP诱导的舒张。苏拉明仅抑制对低剂量腺苷和ATP的反应。8-（对-磺基苯基）茶碱（8-PSPT）在显著抑制对腺苷反应的浓度下，并不影响对ATP和2-meSATP的舒张反应。得出的结论是，2-meSATP通过P2Y嘌呤受体起作用，而ATP似乎主要通过不同机制起作用。它不是通过P1嘌呤受体起作用，因为ATP不受P1嘌呤受体拮抗剂8-PSPT的抑制。
