[嘌呤霉素、萘啶酸和环己酰亚胺对从电鳗（Electrophorus electricus L）纯化的乙酰胆碱酯酶的影响]

[The effect of puromycin, nalidixic acid, and cycloheximide acetylcholinesterase purified from Electrophorus electricus L].

作者信息

Nery da Matta A H, Raul F, Torres da Matta J, Nesralla-Lobão H, Chagas C

出版信息

C R Acad Hebd Seances Acad Sci D. 1975 Sep 8;281(10):655-8.

Abstract

The action of puromycin, nalidixic acid and cycloheximide on acetylcholinesterase has been studied using an "electrophoretically pure" preparation. Results obtained show that these well known inhibitors of protein synthesis have an inhibitory effect on acetylcholinesterase activity. The inhibitory action exercised by puromycin and nalidixic acid is of a mixed inhibition type, similar to the one seen when decamethonium and d-tubocurarine are used as inhibitory agents. Cycloheximide acts in a non competitive way on the enzyme activity.

摘要

利用一种“电泳纯”制剂研究了嘌呤霉素、萘啶酸和放线菌酮对乙酰胆碱酯酶的作用。所得结果表明，这些众所周知的蛋白质合成抑制剂对乙酰胆碱酯酶活性具有抑制作用。嘌呤霉素和萘啶酸所产生的抑制作用属于混合抑制类型，类似于将十烃季铵和d -筒箭毒碱用作抑制剂时所观察到的类型。放线菌酮对该酶的活性起非竞争性作用。