Huang Y, Zeng J, Liu B, Huang A
Hua Xi Yi Ke Da Xue Xue Bao. 1993 Dec;24(4):439-41.
This paper reports the determination of ceftizoxime, a new cephalosporin antibiotic, in serum by RP-HPLC. The technique for serum protein precipitation by adding perchloric acid is adapted for sample preparations. After centrifugation, a 100 microliters portion of aqueous phase is injected into chromatographic column of ultrasphere CN, the mobile phase being a mixture of methanol and 1% acetic acid (15:85). The standard curve is linear within the range of 2.5-120 mg/L in serum. The detection limit of ceftizoxime in serum stands at 0.1 mg/L. The recoveries from serum samples after protein precipitation reach 95.4-96.3%. The within day CVs and interday CVs are 2.7-3.3% and 4.8-7.6%, respectively. This method is rapid, reliable and reproducible. It has been utilized to measure the concentration of ceftizoxime in patient serum for clinical research and pharmacokinetic studies.
本文报道了采用反相高效液相色谱法测定血清中新型头孢菌素抗生素头孢唑肟的含量。采用加入高氯酸进行血清蛋白沉淀的技术进行样品制备。离心后，取100微升水相注入超球CN色谱柱，流动相为甲醇和1%乙酸的混合物（15:85）。血清中头孢唑肟的标准曲线在2.5 - 120 mg/L范围内呈线性。血清中头孢唑肟的检测限为0.1 mg/L。蛋白沉淀后血清样品的回收率为95.4 - 96.3%。日内变异系数和日间变异系数分别为2.7 - 3.3%和4.8 - 7.6%。该方法快速、可靠且可重复。已用于测定患者血清中头孢唑肟的浓度，用于临床研究和药代动力学研究。
