McCormick E M, Echols R M, Rosano T G
Antimicrob Agents Chemother. 1984 Mar;25(3):336-8. doi: 10.1128/AAC.25.3.336.
The application of high-pressure liquid chromatography assays for cephalosporin serum concentrations is difficult in uremic patients because of interference from nondialyzable substances. We developed a high-pressure liquid chromatography method for determining the serum concentration of ceftizoxime in normal and uremic patients. The method involves protein precipitation with acetonitrile, followed by removal of the acetonitrile with dichloromethane. Separation was accomplished with a reverse-phase (C-18) column and a mobile phase of 13% acetonitrile and 2.8% acetic acid. UV detection at 310 nm was used to monitor the peaks. This assay produced a linear relationship between peak height ratio and ceftizoxime concentration from 1.5 to 100 micrograms/ml. Samples from 30 patients were assayed by this method and by a bioassay, with a good correlation of results (r = 0.9832). The method was applicable equally to normal and uremic serum samples.
由于非透析性物质的干扰，高压液相色谱法测定尿毒症患者血清中头孢菌素浓度存在困难。我们开发了一种高压液相色谱法，用于测定正常人和尿毒症患者血清中头孢唑肟的浓度。该方法包括用乙腈进行蛋白质沉淀，然后用二氯甲烷除去乙腈。使用反相（C-18）柱和13%乙腈与2.8%乙酸的流动相进行分离。在310nm处进行紫外检测以监测峰。该测定法在峰高比与头孢唑肟浓度1.5至100微克/毫升之间产生线性关系。用该方法和生物测定法对30例患者的样本进行了测定，结果具有良好的相关性（r = 0.9832）。该方法同样适用于正常和尿毒症血清样本。
