Arkema J M, Schadee-Eestermans I L, Broekhuis-Fluitsma D M, Fukuda M, Hoefsmit E C
Department of Cell Biology, Medical Faculty, Vrije Universiteit, Amsterdam, The Netherlands.
Histochem J. 1994 Feb;26(2):95-9. doi: 10.1007/BF00157956.
In this study, double labelling for major histocompatibility complex (MHC) class I and class II molecules and for MHC molecules and the lysosomal membrane protein lamp-1 on ultrathin cryosections of dendritic cells isolated from human peripheral blood was performed. The plasma membrane proved to be positive for both MHC class I and MHC class II molecules and was labelled for only a very few lamp-1 molecules. MHC class I and MHC class II molecules did not co-localize intracellularly except in some peripherally located vesicles. However, many MHC class II-labelled vesicles were present in a juxtanuclear position but only some of them were co-labelled for lamp-1. These results indicate the presence of a separate, non-lysosomal compartment for class II molecules in dendritic cells.
在本研究中，对从人外周血分离的树突状细胞超薄冷冻切片上的主要组织相容性复合体（MHC）I类和II类分子以及MHC分子和溶酶体膜蛋白lamp-1进行了双重标记。质膜被证明对MHC I类和MHC II类分子均呈阳性，并且仅标记了极少数lamp-1分子。MHC I类和MHC II类分子在细胞内除了在一些位于周边的囊泡中外并不共定位。然而，许多MHC II类标记的囊泡位于核周位置，但其中只有一些与lamp-1共标记。这些结果表明在树突状细胞中存在一个独立的、非溶酶体的II类分子区室。
