de Saint-Vis B, Vincent J, Vandenabeele S, Vanbervliet B, Pin J J, Aït-Yahia S, Patel S, Mattei M G, Banchereau J, Zurawski S, Davoust J, Caux C, Lebecque S
Schering-Plough Laboratory for Immunological Research, Dardilly, France.
Immunity. 1998 Sep;9(3):325-36. doi: 10.1016/s1074-7613(00)80615-9.
We have identified a novel lysosome-associated membrane glycoprotein localized on chromosome 3q26.3-q27, DC-LAMP, which is homologous to CD68. DC-LAMP mRNA is present only in lymphoid organs and DC. A specific MAb detects the protein exclusively in interdigitating dendritic cells. Expression of DC-LAMP increases progressively during in vitro DC differentiation, but sharply upon activation with LPS, TNFalpha, or CD40L. Confocal microscopy confirmed the lysosomal distribution of the protein. Furthermore, DC-LAMP was found in the MHC class II compartment immediately before the translocation of MHC class II molecules to the cell surface, after which it concentrates into perinuclear lysosomes. This suggests that DC-LAMP might change the lysosome function after the transfer of peptide-MHC class II molecules to the surface of DC.
我们鉴定出一种定位于染色体3q26.3 - q27的新型溶酶体相关膜糖蛋白——DC-LAMP，它与CD68同源。DC-LAMP mRNA仅存在于淋巴器官和树突状细胞（DC）中。一种特异性单克隆抗体（MAb）仅在交错突细胞中检测到该蛋白。在体外DC分化过程中，DC-LAMP的表达逐渐增加，但在用脂多糖（LPS）、肿瘤坏死因子α（TNFα）或CD40配体（CD40L）激活后会急剧增加。共聚焦显微镜检查证实了该蛋白的溶酶体分布。此外，在MHC II类分子转运至细胞表面之前，DC-LAMP存在于MHC II类区室中，之后它集中到核周溶酶体中。这表明在肽 - MHC II类分子转移至DC表面后，DC-LAMP可能会改变溶酶体功能。
