Burger D M, Rosing H, Koopman F J, Mennhorst P L, Mulder J W, Bult A, Beijnen J H
Department of Pharmacy, Slotervaart Hospital, Amsterdam, Netherlands.
J Chromatogr. 1993 Dec 22;622(2):235-42. doi: 10.1016/0378-4347(93)80271-5.
A sensitive high-performance liquid chromatographic assay has been developed to determine the levels of 3'-amino-3'-deoxythymidine (AMT), a cytotoxic metabolite of 3'-azido-3'-deoxy-thymidine (AZT, zidovudine), in human plasma. The sample pretreatment involved solid-phase extraction using cation-exchange extraction columns. Chromatography was carried out on a C8 column, using a mobile phase of methanol-0.01 M ammonium acetate (pH 5)-0.25 M sodium dioctylsulfosuccinate (60:40:4, v/v/v) and ultraviolet detection at 265 nm. The method has been validated, and stability tests under various conditions have been performed. The lower limit of quantitation is 5 ng/ml (using 500-microliters human plasma samples). The bioanalytical assay has been used for the determination of AMT in patients with AIDS who used AZT.
已开发出一种灵敏的高效液相色谱分析法，用于测定人血浆中3'-氨基-3'-脱氧胸苷（AMT）的水平，AMT是3'-叠氮-3'-脱氧胸苷（AZT，齐多夫定）的一种细胞毒性代谢产物。样品预处理包括使用阳离子交换萃取柱进行固相萃取。色谱分析在C8柱上进行，流动相为甲醇-0.01M醋酸铵（pH 5）-0.25M二辛基磺基琥珀酸钠（60:40:4，v/v/v），并在265nm处进行紫外检测。该方法已经过验证，并进行了各种条件下的稳定性测试。定量下限为5 ng/ml（使用500微升人血浆样品）。该生物分析方法已用于测定使用AZT的艾滋病患者体内的AMT。
