成年大鼠肝脏和几种莫里斯肝癌中鸟苷酸环化酶的特性
Properties of guanylate cyclase in adult rat liver and several Morris hepatomas.
作者信息
Criss W E, Murad F, Kimura H, Morris H P
出版信息
Biochim Biophys Acta. 1976 Sep 14;445(2):500-8. doi: 10.1016/0005-2744(76)90103-0.
Guanylate cyclase (GTP pyrophyosphate-lyase (cyclizing), EC 4.6.1.2) activity was examined in preparations from normal rat liver and a series of Morris hepatomas. Homogenate gyanylate cyclase activites were 3.2, 1.6 and 1.2 nmol cyclic GMP formed per min/g tissue ihe non-substrate analogs of IMP were weak inhibitors of this enzyme, GMP and four of its analogs had Ki values ranging from 30 to 80 muM. The GMP analogs (8-azaGMP, 7-deaza-8-azaGMP, 2'-dGMP and beta-D-arabinosylGMP) and GMP were competitive inhibitors with respect to GTP.
对来自正常大鼠肝脏和一系列莫里斯肝癌的提取物中的鸟苷酸环化酶（GTP焦磷酸裂解酶（环化），EC 4.6.1.2）活性进行了检测。匀浆鸟苷酸环化酶活性分别为每分钟每克组织形成3.2、1.6和1.2 nmol环鸟苷酸。肌苷一磷酸的非底物类似物是该酶的弱抑制剂，鸟苷一磷酸及其四种类似物的Ki值范围为30至80 μM。鸟苷一磷酸类似物（8-氮杂鸟苷一磷酸、7-脱氮-8-氮杂鸟苷一磷酸、2'-脱氧鸟苷一磷酸和β-D-阿拉伯糖基鸟苷一磷酸）和鸟苷一磷酸是相对于三磷酸鸟苷的竞争性抑制剂。
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