Kimura H, Murad F
Proc Natl Acad Sci U S A. 1975 May;72(5):1965-9. doi: 10.1073/pnas.72.5.1965.
We determined the activities of soluble and particulate guanylate cyclase [GTP pyrophosphatelyase (cyclizing); ?EC 4.6.1.2] IN REGENERATING RAT LIVER, FETAL AND NEONATAL RAT LIVER, AND HEPATOMA. TIn these tissues we found increased particulate and decreased soluble enzyme activities compared to normal adult rat liver. The particulate activity increased 12 hr after partial hepatectomy, reached maximal activity at 48 hr, and then declined. The soluble enzyme activity decreased within 8 hr and continued to decline. The activity of homogenates did not change. Guanylate cyclase activity was increased in plasma membrane and microsome fractions from regenerating liver. The increase in particulate activity was prevented with cycloheximide. Decreased soluble and increased particulate enzyme activities were found in fetal liver. After birth the soluble activity increased and the particulate activity decreased. Seven to 14 days after birth the activities of soluble and particulate fractions were similar to those of adult rat liver. In hepatoma 3924A, the activity of particulate guanylate cyclase was 9-fold greater and that of the soluble enzyme was 50% that of normal liver. These studies suggest that guanylate cyclase activity and its subcellular distribution may be related to liver growth through some unknown mechanism.
我们测定了再生大鼠肝脏、胎鼠和新生大鼠肝脏以及肝癌组织中可溶性和颗粒性鸟苷酸环化酶[GTP焦磷酸裂解酶（环化）；?EC 4.6.1.2]的活性。在这些组织中，与正常成年大鼠肝脏相比，我们发现颗粒性酶活性增加，可溶性酶活性降低。部分肝切除术后12小时颗粒性酶活性增加，48小时达到最大活性，然后下降。可溶性酶活性在8小时内降低，并持续下降。匀浆的活性没有变化。再生肝脏的质膜和微粒体组分中的鸟苷酸环化酶活性增加。放线菌酮可阻止颗粒性酶活性的增加。在胎肝中发现可溶性酶活性降低，颗粒性酶活性增加。出生后，可溶性酶活性增加，颗粒性酶活性降低。出生后7至14天，可溶性和颗粒性组分的活性与成年大鼠肝脏相似。在肝癌3924A中，颗粒性鸟苷酸环化酶的活性比正常肝脏高9倍，可溶性酶的活性为正常肝脏的50%。这些研究表明，鸟苷酸环化酶活性及其亚细胞分布可能通过某种未知机制与肝脏生长有关。
