利用未吸收的兔抗血清检测人类中HLA - DR同种异体抗原特异性的可能性。

Possible detection of HLA-DR alloantigenic specificities in man with unabsorbed rabbit antisera.

作者信息

Solheim B G, Fuks A, Smith L, Strominger J L, Thorsby E

出版信息

Scand J Immunol. 1978;8(1):15-20. doi: 10.1111/j.1365-3083.1978.tb00491.x.

DOI:10.1111/j.1365-3083.1978.tb00491.x

Abstract

Thirty-six rabbits were immunized with precipitin lines formed between detergent solubilized membrane fractions from HLA-D-homozygous cells and a rabbit antiserum to human B cells (anti-p 23/30). Thirty-one of the rabbits produced B-cell specific cytotoxic antibodies and twelve of the antibodies were also precipitating in gel diffusion. In cytotoxicity tests six of the antisera showed clear preferential reactivity with the immunizing HLA-D (DR) antigen. Thus immunization of rabbits might prove valuable for the production of HLA-DR typing reagents.

摘要

用来自HLA-D纯合细胞的去污剂溶解膜组分与兔抗人B细胞抗血清（抗p 23/30）之间形成的沉淀线对36只兔子进行免疫。其中31只兔子产生了B细胞特异性细胞毒性抗体，且其中12种抗体在凝胶扩散中也能沉淀。在细胞毒性试验中，6种抗血清对免疫的HLA-D（DR）抗原有明显的优先反应性。因此，兔免疫法可能对生产HLA-DR分型试剂很有价值。