兔抗高度纯化 HLA - DR 抗原抗血清的反应性。
Reactivity of a rabbit antiserum against highly purified HLA-DR antigens.
作者信息
Klareskog L, Trägårdh L, Lindblom J B, Peterson P A
出版信息
Scand J Immunol. 1978 Mar;7(3):199-208. doi: 10.1111/j.1365-3083.1978.tb00444.x.
A rabbit antiserum has been raised against highly purified, detergent-solubilized HLA-DR antigens. Externally or internally labelled surface glycoproteins from B-lymphocytes, epidermis, macrophages, and B-lymphoma cell lines reacted with the antiserum which precipitated polypeptide chains with the molecular weights 28,000 and 34,000. Such polypeptide chains were not observed when the antiserum was reacted with T-lymphocytes, T-cell lymphoma lines, kidney, brain, thymus and spermatozoa. Fab-fragments of the antiserum completely abolished the cytotoxic action of HLA-DR allantisera but had no effect on the cytotoxicity mediated by HLA-A,B and C loci antisera. Moreover, the rabbit antiserum reacted with the same molecules as the HLA-DR alloantisera. Fab-fragments of the rabbit antiserum completely abolished the MLR response and from the kinetics of the inhibition it is concluded that the Fab-fragments may interfere primarily with the initial recognition phase of the MLR.
已制备出一种针对高度纯化的、经去污剂溶解的HLA - DR抗原的兔抗血清。来自B淋巴细胞、表皮、巨噬细胞和B淋巴瘤细胞系的经外部或内部标记的表面糖蛋白与该抗血清发生反应，该抗血清沉淀出分子量为28,000和34,000的多肽链。当该抗血清与T淋巴细胞、T细胞淋巴瘤系、肾脏、大脑、胸腺和精子发生反应时，未观察到此类多肽链。该抗血清的Fab片段完全消除了HLA - DR同种抗血清的细胞毒性作用，但对由HLA - A、B和C位点抗血清介导的细胞毒性没有影响。此外，兔抗血清与HLA - DR同种抗血清反应的是相同分子。兔抗血清的Fab片段完全消除了混合淋巴细胞反应（MLR），从抑制动力学得出结论，Fab片段可能主要干扰MLR的初始识别阶段。
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