Parkhouse R M, Hunter I R, Abney E R
Immunology. 1976 Mar;30(3):409-12.
Surface IgM on murine splenic lymphocytes was capped using a rhodamine-coupled anti-mu chain system. The cells were then reacted with a polyspecific, fluorescein-coupled anti-mouse immunoglobulin (Ig) under non-capping conditions. A large number (31-41% of total Ig-bearing cells) which gave red caps with anti-mu subsequently stained green peripherally with anti-Fab under the non-capping conditions. The remaining stained cells were divided between those showing only red caps (17-29% of Ig-bearing cells) and those showing only green rings (37-45% of Ig-bearing cells). These data are interpreted by assuming that most of the staining with the second reagent (fluorescein-coupled anti-mouse Ig) is due to the presence of surface associated IgD.
使用罗丹明偶联的抗μ链系统使小鼠脾淋巴细胞上的表面IgM形成帽状。然后在非帽状条件下，将细胞与多特异性、荧光素偶联的抗小鼠免疫球蛋白（Ig）反应。大量（占总Ig阳性细胞的31 - 41%）先用抗μ链产生红色帽状结构的细胞，随后在非帽状条件下用抗Fab在外周染成绿色。其余染色细胞分为仅显示红色帽状结构的细胞（占Ig阳性细胞的17 - 29%）和仅显示绿色环的细胞（占Ig阳性细胞的37 - 45%）。这些数据通过假设第二种试剂（荧光素偶联的抗小鼠Ig）的大多数染色是由于表面相关IgD的存在来解释。
