Miyokawa N
Department of Surgical Pathology, Asahikawa Medical College.
Rinsho Byori. 1994 Mar;42(3):215-7.
Recently striking progress has been made in molecular biology. Among the newly developed techniques, PCR (polymerase chain reaction) is making an epoch in DNA diagnosis in the field of clinical pathology and laboratory medicine. In this symposium, we discussed the available PCR methods in DNA diagnosis and also the future prospect of PCR method as a routine laboratory procedure. First, Dr. Kawabata reviewed the PCR technique and analytical procedures of PCR products. Next five symposium presented the data on DNA diagnosis for particular diseases using the PCR method. Dr. Hasebe presented the HCV genotyping data from the patients. Dr. Hirose showed us the difference in accuracy in DNA typing of Chlamydia trachomatis antigen between the DNA probe method and PCR method. He also introduced the newly developed LCR method. Dr. Kondo reviewed the methods of DNA diagnosis for malignant lymphoma. Dr. Yaginuma presented the analyzed data of tumor suppressor gene p53 in some gynecological tumors. Dr. Azuma presented a case of chronic granulomatosis with a point mutation on the gp91-phox gene which had been revealed by RT-PCR. Next Dr. Yagihashi introduced the protocol for DNA typing of the HLA class II. Finally, Dr. Tanaka presented the availability of the PCR method for the convalescent screening of bone marrow transplantation. We hope this symposium is fruitful and motivating for all staffs of laboratory medicine and pathology. In closing my remarks, I express our appreciation to all symposists as well as Prof. Hisami Ikeda, president of the 27th general meeting of the Hokkaido branch, the Society of Clinical Pathology, planned this symposium.
近年来分子生物学取得了显著进展。在新开发的技术中，聚合酶链反应（PCR）在临床病理学和检验医学领域的DNA诊断方面开创了一个新纪元。在本次研讨会上，我们讨论了DNA诊断中可用的PCR方法以及PCR方法作为常规实验室程序的未来前景。首先，川端博士回顾了PCR技术及PCR产物的分析程序。接下来，五位研讨者展示了使用PCR方法对特定疾病进行DNA诊断的数据。长谷部博士展示了患者的丙型肝炎病毒基因分型数据。广濑博士向我们展示了DNA探针法和PCR法在沙眼衣原体抗原DNA分型准确性上的差异。他还介绍了新开发的连接酶链反应（LCR）方法。近藤博士回顾了恶性淋巴瘤的DNA诊断方法。矢沼博士展示了一些妇科肿瘤中肿瘤抑制基因p53的分析数据。东条博士展示了一例通过逆转录PCR（RT-PCR）发现gp91-噬血细胞氧化酶基因存在点突变的慢性肉芽肿病病例。接下来，矢桥博士介绍了人类白细胞抗原（HLA）Ⅱ类基因分型方案。最后，田中博士介绍了PCR方法在骨髓移植恢复期筛查中的实用性。我们希望本次研讨会对检验医学和病理学的所有工作人员都富有成效且具有启发性。在结束我的发言时，我向所有研讨者以及北海道分会第27届大会主席池田久美教授表示感谢，临床病理学会策划了本次研讨会。
