Kahn A, Pottenger L A, Albertini J G, Taitz A D, Thonar E J
Department of Surgery, University of Chicago, Illinois 60637.
J Surg Res. 1994 Apr;56(4):302-8. doi: 10.1006/jsre.1994.1047.
Severe destruction of articular cartilage in osteoarthritis manifests clinically when repair processes cannot keep up with the catabolic processes. Loss of proteoglycans, which give the tissue its ability to undergo reversible deformation, precedes and probably contributes significantly to breakdown of the matrix in the most superficial layers of articular cartilage. In this study, we have examined the ability of dithiobis[succinimidyl propionate], a bifunctional reagent with a 1.2-nm span that cross-links proteins at lysine amino acid, and poly-L-lysine of high molecular weight (average MW 360,000) to reduce passive loss of proteoglycans and collagen from thin slices (40 and 200 microns) of bovine nasal and human patellar cartilage incubated for 7 days in buffer at 4 degrees C. We present evidence that treatment of thin slices of cartilage with either of these agents is effective in reducing the loss of proteoglycans and collagen from the cartilage matrix and we define conditions (length of treatment and concentrations required) under which the stabilization of the cartilage matrix is optimized. Chemical stabilization of cartilage matrix may become an important modality of treatment in osteoarthritis by protecting the environment around chondrocytes during the repair process.
骨关节炎中关节软骨的严重破坏在修复过程无法跟上分解代谢过程时在临床上表现出来。蛋白聚糖的丢失先于关节软骨最表层基质的破坏，并且可能对其破坏有显著影响，蛋白聚糖赋予组织进行可逆变形的能力。在本研究中，我们检测了二硫代双[琥珀酰亚胺丙酸酯]（一种跨度为1.2纳米、在赖氨酸氨基酸处交联蛋白质的双功能试剂）和高分子量聚-L-赖氨酸（平均分子量360,000）减少牛鼻软骨和人髌软骨薄片（40和200微米）在4℃缓冲液中孵育7天期间蛋白聚糖和胶原蛋白被动丢失的能力。我们提供的证据表明，用这两种试剂中的任何一种处理软骨薄片都能有效减少软骨基质中蛋白聚糖和胶原蛋白的丢失，并且我们确定了使软骨基质稳定性最优化的条件（处理时间和所需浓度）。通过在修复过程中保护软骨细胞周围的环境，软骨基质的化学稳定可能成为骨关节炎治疗的一种重要方式。
