Department of Pharmacology, College of Oriental Medicine, Institute of Oriental Medicine, Kyung Hee University, 1 Hoegi-dong, Dongdaemun-gu, Seoul 130-701, Republic of Korea.
Phytother Res. 2011 Jun;25(6):844-50. doi: 10.1002/ptr.3353. Epub 2010 Nov 19.
Osteoarthritis (OA) is a major degenerative disease affecting millions of individuals. The ability of articular cartilage to self-repair is limited due to a low tissue turnover rate and the avascular nature of the cartilage, making OA an irreversible disease. In Korea, however, many traditional Korean medical doctors have treated joint disease with a prescription of traditional Korean medicine, BaekJeolYuSin-tang (BYT). Thus, the chondroprotective effects of BYT and its active component, berberine (Ber) were investigated in an experimental model. Here it is shown that BYT or Ber significantly inhibited the expression of matrix metalloproteinase (MMP)-3 and a disintegrin and metalloproteinase with thrombospondin motifs-5 as well as increasing the expression of tissue inhibitors of metalloproteinase-1, aggrecan and collagen in rabbit articular chondrocytes (p < 0.05). BYT or Ber significantly inhibited the secretion and activity of MMP-3 (p < 0.05). In addition, BYT or Ber significantly inhibited the release of collagen and glycosaminoglycan into the culture media from rabbit articular cartilage explants (p < 0.05). The data suggest that BYT or Ber has a therapeutic potential for the treatment of cartilage damage in osteoarthritis.
骨关节炎（OA）是一种影响数百万人的主要退行性疾病。由于关节软骨的组织周转率低和软骨的无血管性质，其自我修复能力有限，因此 OA 是一种不可逆转的疾病。然而，在韩国，许多传统的韩国医生用传统的韩国医学处方，百结愈伤汤（BYT）治疗关节疾病。因此，在实验模型中研究了 BYT 及其活性成分小檗碱（Ber）的软骨保护作用。结果表明，BYT 或 Ber 可显著抑制基质金属蛋白酶（MMP）-3 和去整合素金属蛋白酶与血栓反应蛋白-5 的表达，并增加兔关节软骨细胞中金属蛋白酶抑制剂-1、聚集蛋白聚糖和胶原的表达（p<0.05）。BYT 或 Ber 可显著抑制 MMP-3 的分泌和活性（p<0.05）。此外，BYT 或 Ber 可显著抑制兔关节软骨外植体中胶原蛋白和糖胺聚糖释放到培养基中（p<0.05）。数据表明，BYT 或 Ber 具有治疗骨关节炎软骨损伤的潜力。
