Chiba T, Ajito T, Okada K
Department of Veterinary Pathology, Faculty of Agriculture, Iwate University, Morioka, Japan.
Leukemia. 1994 Apr;8 Suppl 1:S206-10.
Distribution of subpopulations of lymphocytes in the lymph nodes and tumor tissues from cattle with enzootic bovine leukosis (EBL) was examined by immunohistology using a panel of monoclonal antibodies against leukocyte differentiation molecules of bovine leukocytes and tumor-associated antigen (TAA) of EBL. As disease progressed in the lymph nodes, TAA positive cells increased in number and were strongly positive. The number of B-B2, IgM positive cells were reduced in frequency in the follicle and areas of neoplastic cell-proliferation. Alpha beta and gamma delta T cells positive for WC1 (workshop cluster designation) were reduced in frequency due to infiltration and proliferation of neoplastic cells. In tumor tissues which were thought to consist with monoclonal population of cells carrying BLV provirus, strongly TAA-positive cells were present throughout the section. Most of the neoplastic cells from five cattle with EBL appeared to be divided into four types, CD2-, CD4-, CD8-, WC1-, B2+ or B2-, IgM+ or IgM-, MHC II+ and strong TAA+. WC1+ cells played no apparent role in the development of lymphosarcoma of EBL. WC1+ cells were not observed in tumor tissues. However, the possibility of effect of T cell for tumor immunity was suggested, since TH/l and Tc/s cells were scattered in the tumor tissues.
利用一组针对牛白细胞分化分子和地方流行性牛白血病（EBL）肿瘤相关抗原（TAA）的单克隆抗体，通过免疫组织学方法检测了患有地方流行性牛白血病（EBL）的牛的淋巴结和肿瘤组织中淋巴细胞亚群的分布情况。随着淋巴结疾病的进展，TAA阳性细胞数量增加且呈强阳性。在卵泡和肿瘤细胞增殖区域，B - B2、IgM阳性细胞的频率降低。由于肿瘤细胞的浸润和增殖，WC1（研讨会集群指定）阳性的αβ和γδ T细胞频率降低。在被认为由携带BLV前病毒的单克隆细胞群体组成的肿瘤组织中，整个切片都存在强TAA阳性细胞。来自五头患有EBL的牛的大多数肿瘤细胞似乎可分为四种类型：CD2 -、CD4 -、CD8 -、WC1 -、B2 +或B2 -、IgM +或IgM -、MHC II +和强TAA +。WC1 +细胞在EBL淋巴肉瘤的发展中未发挥明显作用。在肿瘤组织中未观察到WC1 +细胞。然而，由于TH / l和Tc / s细胞散在于肿瘤组织中，提示了T细胞对肿瘤免疫的影响可能性。
