铜绿假单胞菌耐药因子的演变与作用
Evolution and utility of a Pseudomonas aeruginosa drug resistance factor.
作者信息
Olsen R H, Hansen J
出版信息
J Bacteriol. 1976 Mar;125(3):837-44. doi: 10.1128/jb.125.3.837-844.1976.
Abstract
We describe the addition to the Pseudomonas aeruginosa sex factor, FP2, of carbenicillin resistance encoded by the RP1 plasmid. This occurred in a step-wise manner as detected by variations in the characteristics of the FP2-RP1 plasmid aggregate. The addition of the carbenicillin resistance marker to FP2 facilitates estimates of FP2 transfer. Transfer frequencies for the presumed cointegrate plasmid, using carbenicillin selection, approached 10(-1) per donor bacterium. The chromosomal mobilization properties of the derived plasmid, designated pR0271, resembled those of the progenitor plasmid FP2. Plasmid pR0271 was also observed to mobilize a nontransmissible drug resistance plasmid sharing genetic homology at frequencies corresponding to those observed for chromosomal markers proximal to the origin of transfer.
摘要
我们描述了将由RP1质粒编码的羧苄青霉素抗性添加到铜绿假单胞菌性因子FP2上的情况。这是以逐步的方式发生的，通过FP2 - RP1质粒聚集体特性的变化得以检测。将羧苄青霉素抗性标记添加到FP2上有助于对FP2转移进行评估。使用羧苄青霉素选择法，推测的共整合质粒的转移频率接近每个供体细菌10⁻¹。所衍生的质粒pR0271的染色体动员特性类似于原始质粒FP2的特性。还观察到质粒pR0271能以与转移起点近端的染色体标记所观察到的频率相对应的频率动员具有遗传同源性的非传递性耐药质粒。
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