质粒脱氧核糖核酸对鼠伤寒沙门氏菌的转化
Transformation of Salmonella typhimurium by plasmid deoxyribonucleic acid.
作者信息
Lederberg E M, Cohen S N
出版信息
J Bacteriol. 1974 Sep;119(3):1072-4. doi: 10.1128/jb.119.3.1072-1074.1974.
Abstract
A modified transformation procedure that is effective for the introduction of plasmid deoxyribonucleic acid at high frequency into Salmonella typhimurium, as well as into Escherichia coli, is described. Transformed bacteria acquire a circular deoxyribonucleic acid species having the genetic and molecular characteristics of the transforming plasmid.
摘要
本文描述了一种改良的转化方法，该方法能有效地将质粒脱氧核糖核酸高频导入鼠伤寒沙门氏菌以及大肠杆菌。转化后的细菌获得了一种具有转化质粒遗传和分子特征的环状脱氧核糖核酸。
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