正常人组织中前体-肽血管活性肠肽/PHM-27的基因表达及转录本大小

Gene expression and transcript size of the prepro-peptide VIP/PHM-27 in normal human tissue.

作者信息

Schulte-Bockholt A, Meier D A, Hopp K, Stadelmann A, Otterson M F, Telford G L, Koch T R

机构信息

Division of Gastroenterology, Zablocki VA Medical Center, Milwaukee, WI.

出版信息

Neurosci Lett. 1993 Dec 24;164(1-2):101-4. doi: 10.1016/0304-3940(93)90867-k.

DOI:10.1016/0304-3940(93)90867-k

PMID:8152582

Abstract

The transcript size of VIP/PHM-27 mRNA (vasoactive intestinal peptide/peptide histidine methionine) and the relative distribution of VIP/PHM-27 gene expression in 10 normal human tissues was examined. After mRNA extraction from tissue, VIP/PHM-27 transcript size and relative abundance of mRNA was determined by Northern blot analysis and densitometry of the autoradiograms. VIP/PHM-27 mRNA was detectable in brain, pancreas, colon, ileum and striated muscle while no hybridization signal was observed in liver, kidney, lung, heart, prostate and placental tissue. VIP/PHM-27 transcript in human brain and gut was a single band of 1.7 kb; by contrast, a 7.0-kb transcript was detected in striated skeletal muscle. The highest relative levels of mRNA were observed in brain and pancreas.

摘要

检测了血管活性肠肽/组氨酸甲硫氨酸肽（VIP/PHM - 27）mRNA的转录本大小以及VIP/PHM - 27基因在10种正常人体组织中的相对分布情况。从组织中提取mRNA后，通过Northern印迹分析和放射自显影片的光密度测定法来确定VIP/PHM - 27转录本大小和mRNA的相对丰度。在脑、胰腺、结肠、回肠和横纹肌中可检测到VIP/PHM - 27 mRNA，而在肝、肾、肺、心脏、前列腺和胎盘组织中未观察到杂交信号。人脑中的VIP/PHM - 27转录本是一条1.7 kb的单带；相比之下，在横纹骨骼肌中检测到一条7.0 kb的转录本。在脑和胰腺中观察到最高的mRNA相对水平。