原位杂交显示在猴和大鼠新皮层中存在含血管活性肠肽前体mRNA的神经元。
In situ hybridization reveals VIP precursor mRNA-containing neurons in monkey and rat neocortex.
作者信息
Benson D L, Isackson P J, Jones E G
机构信息
Department of Anatomy and Neurobiology, University of California, Irvine 92717.
出版信息
Brain Res Mol Brain Res. 1991 Jan;9(1-2):169-74. doi: 10.1016/0169-328x(91)90145-n.
The cDNAs encoding monkey vasoactive intestinal polypeptide (VIP) and PHM-27 and rat VIP and PHI-27 were cloned and used to generate antisense RNA probes. Using in situ hybridization, neurons expressing the VIP/PHM or VIP/PHI precursor mRNAs were localized in monkey and rat somatic sensory and visual cortex. In both neocortical areas of both species, labeled cells were observed in all 6 layers as well as the subcortical white matter.
编码猴血管活性肠肽（VIP）和PHM - 27以及大鼠VIP和PHI - 27的cDNA被克隆，并用于生成反义RNA探针。通过原位杂交，在猴和大鼠的躯体感觉皮层和视觉皮层中定位了表达VIP/PHM或VIP/PHI前体mRNA的神经元。在这两个物种的两个新皮层区域中，在所有6层以及皮层下白质中均观察到了标记细胞。
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