前列环素和贝前列素对兔气道上皮纤毛运动的影响。

Effects of prostacyclin and beraprost on ciliary motility of rabbit airway epithelium.

作者信息

Tamaoki J, Sakai S, Chiyotani A, Takeyama K, Tagaya E, Konno K

机构信息

First Department of Medicine, Tokyo Women's Medical College, Japan.

出版信息

Pharmacology. 1994 Mar;48(3):194-200. doi: 10.1159/000139179.

DOI:10.1159/000139179

PMID:8153147

Abstract

The effects of prostacyclin and its stable analog beraprost on ciliary beat frequency (CBF) of rabbit tracheal epithelium were studied by a microphoto-oscillation technique in vitro. Addition of these drugs increased CBF in a dose-dependent fashion: the maximal increase from the baseline value and the drug concentration required to produce a half-maximal effect were 13.3 +/- 2.0% (p < 0.05) and 1.8 +/- 0.4 x 10(-7) mol/l for prostacyclin and 30.8 +/- 3.4% (p < 0.001) and 1.6 +/- 0.5 x 10(-7) mol/l for beraprost. These effects were accompanied by the increase in intracellular cyclic AMP levels. Thus, prostacyclin and beraprost may stimulate ciliary motility and, hence, mucociliary transport through the production of cyclic AMP.

摘要

采用体外显微光振荡技术研究了前列环素及其稳定类似物贝拉前列素对兔气管上皮细胞纤毛摆动频率（CBF）的影响。添加这些药物后，CBF呈剂量依赖性增加：前列环素使CBF从基线值的最大增加幅度和产生半数最大效应所需的药物浓度分别为13.3±2.0%（p<0.05）和1.8±0.4×10⁻⁷mol/L，贝拉前列素分别为30.8±3.4%（p<0.001）和1.6±0.5×10⁻⁷mol/L。这些效应伴随着细胞内环磷酸腺苷（cAMP）水平的升高。因此，前列环素和贝拉前列素可能通过产生cAMP来刺激纤毛运动，从而促进黏液纤毛运输。