Gao T J, Lindholm T S, Marttinen A, Puolakka T
Department of Clinical Medicine, University of Tampere, Finland.
Ann Chir Gynaecol Suppl. 1993;207:77-84.
Using type IV collagen as carrier, the expression of bovine bone morphogenetic protein (bBMP) activity and the relation of ectopic bone formation to BMP dosage in the reconstitution were investigated in BALB mice. Visible heterotopic bone was induced by GuHCl-extracted bovine BMP at a minimal dose of 0.5 mg within 21 days after the BMP was covalently bound to type IV collagen of weight 5.6 mg. The dose-dependent osteogenetic response of BMP was retained in the BMP/type IV collagen composite. As the BMP dose increased in the reconstitution, the integrated intensity and area of bone and cartilage formation, as quantified by a computerized scanner, were enhanced. Degradation of the collagenous carrier was improved by BMP, and neovascularization of the implant site initiated by type IV collagen was also observed. The covalent binding of BMP to type IV collagen postponed the time-sequence of ectopic bone development induced by BMP alone. The conclusion was that the exaggerated and extended effects of type IV collagen on BMP are mainly due to chemotaxis to progenitor cells, immunogenetically inert, vascular initiation and biodegradability in type IV collagen.
以IV型胶原为载体，在BALB小鼠中研究了重组牛骨形态发生蛋白（bBMP）活性的表达以及异位骨形成与BMP剂量在重组中的关系。在BMP与5.6mg重的IV型胶原共价结合后21天内，用盐酸胍提取的牛BMP以最低0.5mg的剂量诱导出可见的异位骨。BMP/IV型胶原复合物保留了BMP的剂量依赖性成骨反应。随着重组中BMP剂量的增加，通过计算机扫描仪量化的骨和软骨形成的积分强度和面积增加。BMP改善了胶原载体的降解，并且还观察到由IV型胶原引发的植入部位的新血管形成。BMP与IV型胶原的共价结合推迟了单独BMP诱导的异位骨发育的时间顺序。结论是IV型胶原对BMP的放大和延长作用主要归因于对祖细胞的趋化性、免疫原性惰性、血管起始以及IV型胶原中的生物降解性。
