Gnudi S, Giardino R, Mongiorgi R, Fini M, Zati A, Figus E, Monti S, Ripamonti C
Rizzoli Orthopaedic Institute, University of Bologna.
Boll Soc Ital Biol Sper. 1993 Jul-Aug;69(7-8):461-8.
The present paper intends to check the possibility of improving convenience of the osteoporosis model from ovariectomy in the rat by anticipating the operation to the 40th week rather than the 52nd week of age, thought by some authors to be the optimum model. To this end two parameters have been examined: 1. the bone mass variation determined with the vertebral photon densitometer and weighing of the femur ashes; 2. the chemico-crystallographic characteristics of the bone determined through chemical analysis and X-ray diffractometry. The results obtained induce us to believe that ovariectomy carried out on rats of 40 or 52 weeks of age determine bone mass losses that are statistically comparable. The variations in the dry bone weight/ash bone weight ratio are superposable and the structural chemical characteristics, due to the increase in bone turnover are testified by the increase in osteocalcin. Moreover, it was observed that the most reliable evaluation of bone mass loss can be obtained with the vertebral photon densitometry. Thus, it is thought that the model which foresees ovariectomy of rats 40 weeks after birth compared with those operated 52 weeks after birth, represents a good model of osteoporosis from estrogen deficiency which, due to the evident practical advantages deriving from the use of younger animals it associates a decrease in the use of laboratory animals with statistically reliable results.
本文旨在探讨将大鼠卵巢切除术骨质疏松模型的手术时机从一些作者认为的最佳年龄52周提前至40周，是否有可能提高该模型的便利性。为此，研究了两个参数：1. 用椎体光子密度计测定并通过称量股骨骨灰确定骨量变化；2. 通过化学分析和X射线衍射法确定骨的化学晶体学特征。所得结果使我们相信，对40周龄或52周龄大鼠进行卵巢切除术导致的骨量损失在统计学上具有可比性。干骨重量/骨灰重量比的变化是可叠加的，骨钙素增加证明了由于骨转换增加导致的结构化学特征。此外，观察到椎体光子密度测定法可获得最可靠的骨量损失评估。因此，与出生后52周进行手术的大鼠相比，出生后40周进行卵巢切除术的模型被认为是雌激素缺乏导致骨质疏松的良好模型，由于使用较年轻动物具有明显的实际优势，它在减少实验动物使用的同时还能获得统计学上可靠的结果。
