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[细针穿刺抽吸在头颈部肿瘤流式细胞术DNA含量测定中的应用。方法与应用]

[Fine-needle puncture-aspiration in the measurement of the DNA content by flow cytometry in head and neck tumors. Methodology and applications].

作者信息

Barona R, O'Connor J E, Comeche C, Montalt J

机构信息

Servicio de ORL, Hospital General Universitario.

出版信息

Acta Otorrinolaringol Esp. 1993 Nov-Dec;44(6):435-8.

PMID:8155358
Abstract

Fine needle aspiration was used to obtain samples from 15 patients with head and neck tumors for posterior analysis of their DNA content by flow cytometry. This technique afforded rapid and readily reproducible high quality histograms. A review was made of the malignancy criteria according to variations in DNA content. Good patient tolerance made it possible to apply the technique repeatedly to obtain samples for DNA analysis by flow cytometry, a method of great interest in monitoring response to different treatment protocols (chemotherapy, radiotherapy).

摘要

采用细针穿刺术从15例头颈部肿瘤患者身上获取样本，以便通过流式细胞术对其DNA含量进行后续分析。该技术能快速生成可重复的高质量直方图。根据DNA含量的变化对恶性肿瘤标准进行了综述。患者耐受性良好，使得该技术能够反复应用以获取样本，用于通过流式细胞术进行DNA分析，这是一种在监测对不同治疗方案（化疗、放疗）的反应方面极具价值的方法。

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