Urano T, Ikeda C, Shimokawa M, Kinoshita T
Department of Analytical Chemistry, School of Pharmaceutical Sciences, Kitasato University, Tokyo, Japan.
Biochim Biophys Acta. 1994 Apr 13;1205(2):258-61. doi: 10.1016/0167-4838(94)90242-9.
We examined the effects of 5,6-trans-prostaglandin E2 (trans-PG E2) on fibrinolysis and plasminogen activation by either urokinase or streptokinase. trans-PG E2 was found to enhance fibrinolysis induced by urokinase and inhibit the one by streptokinase. These effects were also appeared in the method using synthetic chromogenic substrate S-2251, which suggested that the effects of trans-PG E2 were induced in the circumstances without coagulation factors such as fibrinogen, thrombin or fibrin. Moreover, the enhancement effect of trans-PG E2 on fibrinolysis by urokinase was investigated. The result of SDS-PAGE indicated that plasmin formation rate from plasminogen by urokinase was accelerated in the presence of trans-PG E2. As trans-PG E2 increased the hydrolyzing rate of S-2288 by urokinase, trans-PG E2 directly interacted with urokinase. Therefore, the enhancement effect of trans-PG E2 on plasminogen activation by urokinase could be explained, at least in part, as follows: at first trans-PG E2 directly exerts its effect on urokinase, then it causes the increase of generation rate of plasmin from plasminogen.
我们研究了5,6-反式前列腺素E2（反式-PG E2）对纤溶以及由尿激酶或链激酶引起的纤溶酶原激活的影响。发现反式-PG E2可增强尿激酶诱导的纤溶作用，并抑制链激酶诱导的纤溶作用。在使用合成显色底物S-2251的方法中也出现了这些效应，这表明反式-PG E2的效应是在没有纤维蛋白原、凝血酶或纤维蛋白等凝血因子的情况下诱导产生的。此外，还研究了反式-PG E2对尿激酶纤溶作用的增强效应。SDS-PAGE结果表明，在反式-PG E2存在的情况下，尿激酶使纤溶酶原形成纤溶酶的速率加快。由于反式-PG E2提高了尿激酶对S-2288的水解速率，所以反式-PG E2直接与尿激酶相互作用。因此，反式-PG E2对尿激酶激活纤溶酶原的增强效应至少可以部分解释如下：首先，反式-PG E2直接对尿激酶发挥作用，然后导致纤溶酶原产生纤溶酶的速率增加。
