Yamada T, Mishima T, Ishizaki M, Shida T, Iikura Y
Department of Pediatrics, Atsugi Prefectural Hospital, Kanagawa, Japan.
Int Arch Allergy Immunol. 1994;104 Suppl 1(1):2-5. doi: 10.1159/000236734.
To investigate the condition of peripheral eosinophils in patients with bronchial hyperresponsiveness, we examined density distribution profiles and ultrastructure of peripheral eosinophils obtained before and after exercise challenge test. Following exercise challenge test, the number of hypodense eosinophils (< 1.0825 g/ml) was significantly higher in the EIA-positive than in the EIA-negative subjects. Regarding ultrastructural analysis, the ratio of the area of specific granules to the area of cytoplasm (SG/C) per cell was significantly higher in the EIA-negative than in the EIA-positive patients. The ratio of the degranulated area to the area of cytoplasm (D/C) per cell was significantly higher in the EIA-positive than in the EIA-negative subjects. These data suggested that activation of eosinophils in the patients with bronchial hyperresponsiveness occurs during the preinflammatory phase.
为研究支气管高反应性患者外周血嗜酸性粒细胞的状况，我们检测了运动激发试验前后外周血嗜酸性粒细胞的密度分布情况及超微结构。运动激发试验后，运动诱发哮喘（EIA）阳性受试者中低密度嗜酸性粒细胞（< 1.0825 g/ml）的数量显著高于EIA阴性受试者。关于超微结构分析，EIA阴性患者单个细胞中特异性颗粒面积与细胞质面积之比（SG/C）显著高于EIA阳性患者。EIA阳性受试者单个细胞中脱颗粒面积与细胞质面积之比（D/C）显著高于EIA阴性受试者。这些数据表明，支气管高反应性患者嗜酸性粒细胞的激活发生在炎症前期。
