Lessin S R, Rook A H
Department of Dermatology, University of Pennsylvania, Philadelphia.
Exp Dermatol. 1993 Mar;2(2):53-62. doi: 10.1111/j.1600-0625.1993.tb00009.x.
The growth of our knowledge in T-cell biology, in particular the molecular biology of the T-cell receptor (TCR), has provided a means to molecularly characterize lymphoproliferative diseases of the skin based on the presence or absence of a clonal population of T lymphocytes. TCR gene rearrangement studies, by Southern blot analysis, have aided the investigative dermatologist in gaining insights into the pathogenesis and clonal evolution of lymphoproliferative skin diseases. In addition, the application of TCR gene rearrangement studies as a diagnostic aid in the evaluation of lymphoproliferative skin diseases has been introduced into clinical dermatology. Despite its enormous research value, TCR gene rearrangement studies presently have limited applications as an independent diagnostic tool. However, as our knowledge and experience grows and as the application of new techniques provides us with greater detection sensitivity and specificity, the diagnostic utility of TCR gene rearrangement studies will be enhanced.
我们在T细胞生物学领域，特别是T细胞受体（TCR）分子生物学方面的知识增长，为基于T淋巴细胞克隆群体的有无对皮肤淋巴增殖性疾病进行分子特征描述提供了一种方法。通过Southern印迹分析进行的TCR基因重排研究，有助于皮肤科研究人员深入了解淋巴增殖性皮肤病的发病机制和克隆演变。此外，TCR基因重排研究作为评估淋巴增殖性皮肤病的诊断辅助手段已被引入临床皮肤科。尽管TCR基因重排研究具有巨大的研究价值，但目前作为独立诊断工具的应用有限。然而，随着我们知识和经验的增加，以及新技术的应用为我们提供更高的检测灵敏度和特异性，TCR基因重排研究的诊断效用将会提高。
