利血平化大鼠脑海马和纹状体中的蛋白激酶C亚型

Protein kinase C subspecies in hippocampus and striatum of reserpinized rat brain.

作者信息

Komachi H, Yanagisawa K, Shirasaki Y, Miyatake T

机构信息

Department of Neurology, Tokyo Medical and Dental University, Japan.

出版信息

Brain Res. 1994 Jan 14;634(1):127-30. doi: 10.1016/0006-8993(94)90265-8.

DOI:10.1016/0006-8993(94)90265-8

PMID:8156381

Abstract

Protein kinase C (PKC) (alpha) and (beta) immunoreactivities were determined in the cytosolic and particulate fractions of the hippocampus and striatum after single and repetitive, intraperitoneal administration of reserpine. Significant decrease of PKC(alpha) immunoreactivities was observed in the particulate fractions of both striatum and hippocampus. Compared with the alteration of PKC(alpha), the decreased immunoreactivity of PKC(beta) was only observed in the particulate fraction of the hippocampus, associated with the increase in the cytosolic fraction. These findings suggest a novel mechanism regulating the distribution of PKC. The topographical selectivity in the present study may indicate the importance of the alteration of PKC(beta) in the pathophysiological mechanism in Alzheimer's disease.

摘要

单次及重复腹腔注射利血平后，测定海马体和纹状体胞质和微粒体部分中的蛋白激酶C（PKC）（α）和（β）免疫反应性。纹状体和海马体的微粒体部分中均观察到PKC（α）免疫反应性显著降低。与PKC（α）的变化相比，PKC（β）免疫反应性降低仅在海马体的微粒体部分观察到，且与胞质部分的增加相关。这些发现提示了一种调节PKC分布的新机制。本研究中的拓扑选择性可能表明PKC（β）的改变在阿尔茨海默病病理生理机制中的重要性。