不同志贺氏菌属细菌产生的一种与霍乱弧菌相关的肠毒素。
A cholera-coli related enterotoxin production by different Shigella species.
作者信息
Panigrahi D, Nayak N, Ganguly N K, Roy P, Sehgal R
机构信息
Department of Medical Microbiology and Experimental Medicine, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
出版信息
Indian J Pathol Microbiol. 1993 Oct;36(4):403-9.
A cholera-coli related enterotoxin production was studied in 50 different Shigella isolates from cases of childhood diarrhoea. Four out of 6 Sh. dysenteriae, 18/37 Sh. flexneri and 2/4 Sh. sonnei were found to be enterotoxin producers by RIL test. All strong RIL positive strains were isolated from cases of severe diarrhoea, indicating the association of enterotoxin production and severity of acute diarrhoea. Two major protein bands were observed in SDS-PAGE and W.B. EIA assay in all positive RIL extracts. These immuno-reactive bands were at 31 kDa and 14 kDa positions resembling A-B subunit structure of cholera-coli family of enterotoxins.
对50株来自儿童腹泻病例的不同志贺氏菌分离株进行了霍乱大肠杆菌相关肠毒素产生情况的研究。通过反向间接血凝试验（RIL试验）发现，6株痢疾志贺氏菌中有4株、37株福氏志贺氏菌中有18株、4株宋内氏志贺氏菌中有2株为肠毒素产生菌。所有RIL强阳性菌株均分离自严重腹泻病例，表明肠毒素产生与急性腹泻的严重程度有关。在所有RIL阳性提取物的十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳（SDS - PAGE）和免疫印迹酶免疫分析（W.B. EIA）试验中观察到两条主要蛋白带。这些免疫反应带位于31 kDa和14 kDa位置，类似于霍乱大肠杆菌家族肠毒素的A - B亚基结构。
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