Simón C, Frances A, Piquette G, Hendrickson M, Milki A, Polan M L
Department of Gynecology and Obstetrics, Stanford University Medical Center, California 94305.
J Clin Endocrinol Metab. 1994 Apr;78(4):847-54. doi: 10.1210/jcem.78.4.8157710.
Interleukin-1 receptor type I, IL-1 beta, IL-1 receptor antagonist, and human macrophages were immunohistochemically localized in the villous trophoblast, maternal-trophoblast interphase, and maternal decidua during early human implantation. Immunostaining for IL-1 receptor type I was present in the syncytiotrophoblast and hyperplastic endometrial glands in the maternal decidua. Immunoreactive IL-1 beta was present in the villous cytotrophoblast, syncytiotrophoblast, intermediate trophoblast, and maternal stromal decidual cells. IL-1 receptor antagonist staining was observed in the glandular endometrium of the maternal decidua and in isolated cells located inside the chorionic villi, intervillous space, and maternal decidua. Mature human macrophages, as defined by both CD/68+ and HAM56+, were present in the chorionic villi, maternal blood of intervillous space, and maternal decidua. Co-localization studies demonstrated that macrophages in all of the reported locations also stained for immunoreactive IL-1 beta. Our results show the shared presence in maternal and embryonic tissues of this receptor-agonist-antagonist system during early human implantation. This finding supports an autocrine/paracrine role for the IL-1 system in human implantation.
在人类早期着床过程中，通过免疫组织化学方法对I型白细胞介素-1受体、白细胞介素-1β、白细胞介素-1受体拮抗剂和人类巨噬细胞在绒毛滋养层、母-胎滋养层界面及母体蜕膜中进行了定位。I型白细胞介素-1受体的免疫染色出现在合体滋养层和母体蜕膜中增生的子宫内膜腺体中。免疫反应性白细胞介素-1β出现在绒毛细胞滋养层、合体滋养层、中间滋养层及母体基质蜕膜细胞中。在母体蜕膜的腺状子宫内膜以及位于绒毛膜绒毛内、绒毛间隙和母体蜕膜中的单个细胞中观察到白细胞介素-1受体拮抗剂染色。由CD68+和HAM56+定义的成熟人类巨噬细胞存在于绒毛膜绒毛、绒毛间隙的母体血液及母体蜕膜中。共定位研究表明，在所有报道位置的巨噬细胞也对免疫反应性白细胞介素-1β呈染色阳性。我们的结果显示，在人类早期着床过程中，该受体-激动剂-拮抗剂系统在母体和胚胎组织中共同存在。这一发现支持白细胞介素-1系统在人类着床过程中具有自分泌/旁分泌作用。
