Mawn J A, Simpson A J, Heard S R
Department of Medical Microbiology, St Bartholomew's Hospital, West Smithfield, London.
J Clin Pathol. 1993 Jul;46(7):633-6. doi: 10.1136/jcp.46.7.633.
To develop a polymerase chain reaction (PCR) for the specific detection of the C protein gene in strains of group B Streptococcus.
A single primer pair derived from the nucleotide sequence of the IgA binding beta antigen of the C protein complex permitted the specific amplification of a 592 base pair DNA fragment from the C protein gene. After 35 cycles of amplification this product could be detected by agarose gel electrophoresis. Southern blot hybridisation confirmed that this product was the C protein gene.
PCR detected the C protein gene in 75 (63%) of 119 strains of group B streptococci analysed. The product was not detected in other Gram positive organisms, showing that this PCR assay was highly specific. The sensitivity of the assay was satisfactory to a dilution of 1 in 10,000 of extracted DNA.
The C protein of group B streptococci is associated with neonatal sepsis. The specific detection of the C protein gene by PCR may help identify which strains are likely to be associated with infection by the organism.
开发一种用于特异性检测B族链球菌菌株中C蛋白基因的聚合酶链反应（PCR）。
一对源自C蛋白复合物IgA结合β抗原核苷酸序列的引物，可特异性扩增出C蛋白基因的一段592碱基对的DNA片段。经过35个循环的扩增后，该产物可通过琼脂糖凝胶电泳检测到。Southern印迹杂交证实该产物为C蛋白基因。
PCR在分析的119株B族链球菌中的75株（63%）中检测到了C蛋白基因。在其他革兰氏阳性菌中未检测到该产物，表明该PCR检测具有高度特异性。该检测方法对提取DNA稀释至1/10000的灵敏度令人满意。
B族链球菌的C蛋白与新生儿败血症有关。通过PCR特异性检测C蛋白基因可能有助于识别哪些菌株可能与该病原体感染有关。
