Heard S R, Mawn J A
Department of Medical Microbiology, St Bartholomew's Hospital Medical College, West Smithfield, London.
J Clin Pathol. 1993 Feb;46(2):145-8. doi: 10.1136/jcp.46.2.145.
To develop a new typing system for group B streptococci based on 35S-methionine-labelled protein profiles of bacterial proteins.
377 clinical isolates of group B streptococci were examined by incorporation of 35S-methionine into bacterial proteins under strict anaerobic conditions. After sodium dodecylsulphate-polyacrylamide gel electrophoresis, autoradiography was performed. The patterns produced were visually analysed and categorised into clusters of organisms based on the pattern of band production between 32-46 kilodaltons.
294 of the typed strains classified into seven different groups designated a-g. 32 strains failed to incorporate 35S-methionine sufficiently to be grouped and 11 strains did not fall into one of the seven identified groups. Typability, reproducibility, and discrimination of the system was evident.
This typing system may help to distinguish between colonising and invasive strains of the organism.
基于用³⁵S-甲硫氨酸标记的细菌蛋白质谱，开发一种用于B族链球菌的新型分型系统。
在严格厌氧条件下，通过将³⁵S-甲硫氨酸掺入细菌蛋白质中，对377株B族链球菌临床分离株进行检测。进行十二烷基硫酸钠-聚丙烯酰胺凝胶电泳后，进行放射自显影。对产生的图谱进行视觉分析，并根据32 - 46千道尔顿之间的条带产生模式将生物体分类为不同的簇。
294株分型菌株分为七个不同的组，命名为a - g。32株菌株未能充分掺入³⁵S-甲硫氨酸而无法分组，11株菌株不属于七个已确定的组之一。该系统的分型能力、可重复性和鉴别能力很明显。
这种分型系统可能有助于区分该生物体的定植菌株和侵袭性菌株。
