Wahlberg J, Fiore J, Angarano G, Uhlén M, Albert J
Department of Biochemistry and Biotechnology, Royal Institute of Technology, Stockholm, Sweden.
J Infect Dis. 1994 Mar;169(3):611-4. doi: 10.1093/infdis/169.3.611.
The sexual transmission of zidovudine-resistant human immunodeficiency virus type 1 (HIV-1) variants was investigated in 5 donor-recipient pairs in which all donors and none of the recipients had received zidovudine treatment. The virus isolates were tested for sensitivity to zidovudine (IC50) in vitro using blood donor lymphocytes. A region of the HIV-1 pol gene was also directly sequenced by a solid-phase sequencing method. Four donors were shown to have zidovudine-resistant HIV-1 variants. Two of these patients had a single mutation (Thr215-->Tyr), and 2 had a double mutation (Met41-->Leu and Thr215-->Tyr) that previously has been shown to confer zidovudine resistance. Zidovudine-resistant virus was found in only 1 of the 4 recipients, which indicates that zidovudine-resistant HIV-1 variants may be selected against during transmission. Thus, the transmission of zidovudine-resistant HIV-1 variants is a complex process that will require consideration whenever zidovudine treatment is initiated in persons who may have been infected by resistant variants.
对5对供体-受体进行了齐多夫定耐药的1型人类免疫缺陷病毒（HIV-1）变异株性传播的研究，其中所有供体均未接受过齐多夫定治疗，而所有受体均未接受过该治疗。使用献血者淋巴细胞在体外检测病毒分离株对齐多夫定的敏感性（IC50）。还通过固相测序方法直接对HIV-1 pol基因的一个区域进行了测序。结果显示4名供体携带齐多夫定耐药的HIV-1变异株。其中2名患者有一个单一突变（Thr215→Tyr），2名患者有一个双重突变（Met41→Leu和Thr215→Tyr），先前已证明该双重突变可导致对齐多夫定耐药。在4名受体中仅1名发现了齐多夫定耐药病毒，这表明齐多夫定耐药的HIV-1变异株在传播过程中可能会被淘汰。因此，齐多夫定耐药的HIV-1变异株的传播是一个复杂的过程，在可能已被耐药变异株感染的人群开始使用齐多夫定治疗时，这一过程都需要加以考虑。
