Albert J, Wahlberg J, Leitner T, Escanilla D, Uhlén M
Department of Clinical Virology, Swedish Institute for Infectious Disease Control, Karolinska Institute, Stockholm.
J Virol. 1994 Sep;68(9):5918-24. doi: 10.1128/JVI.68.9.5918-5924.1994.
Transmission of human immunodeficiency virus type 1 (HIV-1) from a male accused of rape and deliberate transmission of HIV-1 was investigated by sequencing of the HIV-1 pol and gag genes from virus obtained from the male and from the female victim. Parts of the reverse transcriptase and p17gag genes were amplified and directly sequenced from uncultured peripheral blood mononuclear cells. The sequences were compared with sequences from 21 unrelated HIV-1-infected controls from the same geographic area (Stockholm, Sweden). Bootstrap analysis of phylogenetic trees demonstrated that the sequences from the female were significantly more closely related to the sequences from the male than to sequences from the controls. Furthermore, we found that the male and female shared two distinct genetic variants of HIV-1. In p17gag the major variant had an unusual, out-of-frame deletion of 3 nucleotides which the minor variant lacked. These results indicated that the male had transmitted more than one infectious unit to the female. From this study we concluded that it was highly likely that the HIV-1 strains carried by the male and female were closely epidemiologically linked.
对一名被指控强奸并蓄意传播1型人类免疫缺陷病毒（HIV-1）的男性所传播的HIV-1进行了调查，通过对从该男性和女性受害者身上获取的病毒的HIV-1 pol和gag基因进行测序。从未经培养的外周血单个核细胞中扩增出部分逆转录酶和p17gag基因并直接测序。将这些序列与来自同一地理区域（瑞典斯德哥尔摩）的21名不相关的HIV-1感染对照的序列进行比较。对系统发育树的自展分析表明，女性的序列与男性的序列相比，与对照的序列关系明显更密切。此外，我们发现男性和女性共享两种不同的HIV-1基因变体。在p17gag中，主要变体有一个不寻常的、移码的3个核苷酸缺失，而次要变体没有。这些结果表明，男性向女性传播了不止一个感染单位。从这项研究中我们得出结论，男性和女性携带的HIV-1毒株在流行病学上很可能密切相关。
