Silva J, Tang Y J, Gumerlock P H
Department of Internal Medicine, School of Medicine, University of California, Davis Medical Center, Sacramento 95817.
J Infect Dis. 1994 Mar;169(3):661-4. doi: 10.1093/infdis/169.3.661.
Arbitrarily primed polymerase chain reaction (AP-PCR) was used to genotype Clostridium difficile isolates from various sources. Four major molecular types were identified among strains from the American Type Culture Collection previously typed by serogroup and from isolates from patients at the University of California, Davis Medical Center, from a patient at a Utah institution, and from the environment. These groups contained subgroups that displayed, in addition to the common group bands, at least one unique band. Two strains isolated from patients at our institution had the same DNA banding patterns. These patients were hospitalized during the same period, raising the possibility of cross-infection through hospital contact or another common source. These results suggest that this AP-PCR approach will be useful in epidemiologic studies of C. difficile infections.
采用任意引物聚合酶链反应（AP-PCR）对来自不同来源的艰难梭菌分离株进行基因分型。在美国典型培养物保藏中心先前按血清群分型的菌株、加利福尼亚大学戴维斯分校医学中心患者的分离株、犹他州某机构一名患者的分离株以及环境分离株中，鉴定出了四种主要分子类型。这些组包含的亚组除了具有共同的组带外，还至少有一条独特的带。从我们机构的患者中分离出的两株菌株具有相同的DNA条带模式。这些患者在同一时期住院，增加了通过医院接触或其他共同来源发生交叉感染的可能性。这些结果表明，这种AP-PCR方法将有助于艰难梭菌感染的流行病学研究。
