Collier M C, Stock F, DeGirolami P C, Samore M H, Cartwright C P
Clinical Pathology Department Warren G. Magnuson Clinical Center, National Institutes of Health, Bethesda, Maryland.
J Clin Microbiol. 1996 May;34(5):1153-7. doi: 10.1128/jcm.34.5.1153-1157.1996.
Representative isolates of the 10 serogroups of Clostridium difficile and 39 clinical isolates (30 toxigenic and 9 nontoxigenic), including 5 isolates from a confirmed nosocomial outbreak, were analyzed by using two previously described arbitrary-primer PCR (AP-PCR) molecular typing methodologies (AP-PG05 and AP-ARB11) and PCR ribotyping. The two AP-PCR methods investigated gave comparable results; AP-PG05 and AP-ARB11 identified 8 and 7 groups among the serogroup isolates and classified the clinical isolates into 21 and 20 distinct groups, respectively. PCR ribotyping also identified 8 unique groups among the serogroup isolates but classified the clinical isolates into 23 groups. In addition, when results obtained by the PCR methods were compared with typing data generated by pulsed-field gel electrophoresis (PFGE), PCR ribotyping and PFGE were found to be in agreement for 83% (29 of 35) of isolates typeable by both techniques while AP-PG05 was in agreement with PFGE for 60% (20 of 33) and AP-ARB11 was in agreement with PFGE for only 44% (17 of 36). These results indicate that PCR ribotyping is a more discriminatory approach than AP-PCR for typing C. difficile and, furthermore, that this technique generates results that are in higher concordance with those obtained by using an established method for differentiating isolates of this organism on a molecular level than are results generated by using AP-PCR.
采用两种先前描述的任意引物PCR（AP-PCR）分子分型方法（AP-PG05和AP-ARB11）以及PCR核糖体分型法，对艰难梭菌10个血清型的代表性菌株和39株临床分离株（30株产毒菌株和9株无毒菌株）进行了分析，其中包括5株来自确诊医院感染暴发的分离株。所研究的两种AP-PCR方法得出了相似的结果；AP-PG05和AP-ARB11在血清型菌株中分别鉴定出8个和7个组，并将临床分离株分别分为21个和20个不同的组。PCR核糖体分型在血清型菌株中也鉴定出8个独特的组，但将临床分离株分为23个组。此外，当将PCR方法获得的结果与脉冲场凝胶电泳（PFGE）产生的分型数据进行比较时，发现PCR核糖体分型和PFGE对于两种技术均可分型的分离株中有83%（35株中的29株）结果一致，而AP-PG05与PFGE的一致率为60%（33株中的20株），AP-ARB11与PFGE的一致率仅为44%（36株中的17株）。这些结果表明，对于艰难梭菌的分型，PCR核糖体分型比AP-PCR更具鉴别力，而且与使用AP-PCR相比，该技术所产生的结果与使用一种已确立的在分子水平上区分该微生物分离株的方法所获得的结果具有更高的一致性。
